Enzymatic synthesis of Beta-Nicotinamide Mononucleotide (NMN) :greener and more efficient

- Mar 10, 2021-

The researchers used AMP and nucleosidase, PRS and NAMPT to synthesize Beta-Nicotinamide Mononucleotide (NMN) using phosphoric acid, nicotinamide, and inosinic acid as substrates. The reaction has a high conversion rate, but the AMP nucleosidase used is relatively less researched, it is difficult to find an enzyme suitable for production using this process.

They used ribokinase, ribose phosphopyrophosphate kinase, and NAMPT multiple enzymes to synthesize NMN using nicotinamide, adenosine triphosphate and ribose as raw materials.

Beta-Nicotinamide Mononucleotide (NMN).jpg

The reaction feeding method can be a one-step or a distributed method (first ribose reacts with adenosine triphosphate to obtain PRPP, and then nicotinamide is added to obtain NMN). The advantages of One-step feeding short reaction time and simple operation, while distributed is a thorough reaction. The reaction conversion rate can also reach 80%-100%, the study of ribokinase and ribose phosphopyrophosphate kinase is depth, the reaction process is clear.

In 2018, Zhu Wei and others used D-5-phosphoribose and nicotinamide as raw materials, in the presence of ATP, solidified whole-cell catalysis of genetically engineered bacteria containing phosphoribose pyrophosphate synthase and NAMPT to achieve efficient Beta-Nicotinamide Mononucleotide (NMN) synthesis in one step , NMN concentration can reach 13.3g/L, the conversion rate can reach 99.5%. The process has many advantages such as low cost, simple production process, recyclable cells, etc., which has certain effects in cost saving.

used AMP and nucleosidase, PRS and NAMPT to synthesize Beta-Nicotinamide Mononucleotide (NMN)

used D-5-phosphoribose and nicotinamide as raw materials

It can be seen that researchers have been developing better processes for Beta-Nicotinamide Mononucleotide (NMN). Enzyme-catalyzed synthesis, as a green and efficient synthesis method, plays a pivotal role in the preparation of NMN. It is certain that with the continuous improvement of biotechnology and the deepening of research on the NMN enzyme catalytic process, more excellent NMN enzyme catalytic processes will continue to emerge in the future.

greener and more efficient

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